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Published on: May 9, 2020
A validated assay for the quantitative analysis of tranexamic acid in human serum by liquid chromatography coupled
Stanislas Grassin Delyle1, Emuri Abe, Anne Batisse
1AP-HP, Hôpital Raymond Poincaré, Laboratory of Pharmacology-Toxicology, Garches, France. s.grassindelyle@gmail.com
Background:
Tranexamic acid is a synthetic lysine analog used for management of bleeding disorders. The objective of this study was first to develop a method for measurement of tranexamic acid in human serum using liquid chromatography coupled to ion-trap mass spectrometry (LC-MS/MS), and then to validate it throughout a wide range of concentrations allowing quantification in patients receiving tranexamic acid infusion during surgery.
Methods:
Serum samples (100 microL) were subjected to protein precipitation with perchloric acid, and after pH adjustment, tranexamic acid and internal standard were separated on a C(18) column and isocratically eluted using a mobile phase constituted of formate buffer/acetonitrile (95:5, v/v). Tranexamic acid was ionized by electrospray in positive mode. Parent [M+H](+) ions were m/z 158.0 for tranexamic acid and m/z 144.0 for IS. The most intense product ion of tranexamic acid (m/z 122.7) and IS (m/z 126.0) were used for quantification.
Results:
The assay was accurate and precise over the range of 1.0 (lower limit of quantification) to 200.0 microg/mL (upper limit of quantification), and has been successfully applied to study the clinical pharmacokinetics in two volunteers undergoing cardiac surgery.
Conclusion:
A reliable method for quantification of tranexamic acid for analysis in clinical studies was obtained.
