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Updated: Jun 17, 2026

Single Cell Transfection in Chick Embryos
08:46

Single Cell Transfection in Chick Embryos

Published on: September 25, 2010

Single cell optical transfection.

David J Stevenson1, Frank J Gunn-Moore, Paul Campbell

  • 1SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, UK. ds50@st-andrews.ac.uk

Journal of the Royal Society, Interface
|January 13, 2010
PubMed
Summary
This summary is machine-generated.

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Optical transfection uses lasers to create temporary pores in cell membranes, enabling the precise intracellular delivery of molecules like DNA and RNA. This contactless method offers a powerful new tool for cell biology research and applications.

Area of Science:

  • Cell Biology
  • Biophysics
  • Nanoscience

Background:

  • Eukaryotic cells possess plasma membranes impermeable to most hydrophilic substances, hindering intracellular delivery.
  • Traditional transfection methods (viral, lipoplex, electroporation) have limitations.
  • Cell biologists require efficient methods for intracellular delivery of reagents and nanoscopic objects.

Purpose of the Study:

  • To explore optical injection and optical transfection as novel methods for intracellular delivery.
  • To review the principles and applications of optical transfection.
  • To forecast future potential of this technology.

Main Methods:

  • Optical injection utilizes a laser field to transiently increase membrane permeability.
  • Optical transfection specifically refers to the intracellular delivery of nucleic acids (DNA, RNA, siRNA) via optical injection.

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Last Updated: Jun 17, 2026

Single Cell Transfection in Chick Embryos
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Published on: September 25, 2010

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
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An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

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Rapid Development of Cell State Identification Circuits with Poly-Transfection

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  • This contactless, aseptic, single-cell method acts as a nanosurgical tool.

    • Main Results:

    • Optical injection allows for the internalization of various substances into cells.
    • Optical transfection enables precise delivery of nucleic acids into single cells.
    • The technology facilitates intracellular delivery of reagents and nanoscopic objects.

    Conclusions:

    • Optical transfection is a promising contactless and aseptic method for single-cell transfection.
    • This technique offers significant potential for cell biology research and intracellular delivery applications.
    • Further experimental possibilities and applications of optical transfection are anticipated.