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Updated: Jun 17, 2026

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Published on: December 18, 2015

Mapping lineage using BAC-Cre reporter lines.

Qing Xu¹, Stewart A Anderson

¹Weill Medical College of Cornell University, New York, New York, USA.

Current Protocols in Neuroscience

|January 13, 2010

Summary

This summary is machine-generated.

Bacterial artificial chromosome (BAC) technology enables precise lineage tracing in the developing brain. BAC-Cre mice crossed with reporter mice allow permanent marking of progenitor cell fates for developmental studies.

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RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

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Published on: May 19, 2019

Area of Science:

Neuroscience
Developmental Biology
Genetics

Background:

Progenitor cells differentiate into specific neuronal and glial subtypes during brain development.
Understanding cell lineage is crucial for studying brain development and disorders.

Purpose of the Study:

To present a versatile method for tracing progenitor cell lineages into adult cell types using bacterial artificial chromosome (BAC)-Cre transgenic mice.
To detail experimental procedures for BAC-Cre mouse generation and their application in fate-mapping studies.

Main Methods:

Utilizing Cre recombinase targeted to a gene's promoter via a bacterial artificial chromosome (BAC) for lineage tracing.
Employing Cre-loxP recombination to permanently activate reporter genes (e.g., GFP) in progenitor cells and their progeny.
Describing RecA-based BAC DNA modification and pronuclear injection techniques.

Main Results:

BAC-Cre mice accurately reflect endogenous gene expression patterns due to retained regulatory elements.
Cre-loxP recombination in crossed reporter mice permanently labels all cells derived from the targeted progenitor.
This method allows for reliable fate-mapping of neuronal and glial lineages.

Conclusions:

BAC-Cre transgenic mice offer a robust and reliable system for lineage tracing in neurodevelopmental studies.
The described methodology facilitates detailed analysis of progenitor cell behavior and fate determination.