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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 17, 2026

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth
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Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth

Published on: November 10, 2023

Practical issues in high-speed cell sorting.

Larry W Arnold1, Joanne Lannigan

  • 1The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Current Protocols in Cytometry
|January 14, 2010
PubMed
Summary
This summary is machine-generated.

High-speed cell sorting utilizes advanced electronics and data processing for rapid cell analysis. Achieving optimal results requires precise implementation of mechanical, software, and cell preparation factors.

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Related Experiment Videos

Last Updated: Jun 17, 2026

Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth
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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)
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Purification of Specific Cell Population by Fluorescence Activated Cell Sorting (FACS)

Published on: July 10, 2010

Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Modern flow cytometers offer "high-speed sorting" capabilities.
  • There is ambiguity regarding the definition and practical limits of "high-speed" cell sorting.
  • Achieving high-speed sorting necessitates careful consideration of multiple parameters for optimal outcomes.

Purpose of the Study:

  • To clarify the capabilities and considerations for high-speed cell sorting.
  • To discuss the factors influencing sorting speed, purity, recovery, and cell functionality.
  • To provide a comprehensive overview of high-speed cell sorting theory and practice.

Main Methods:

  • Discussion of theoretical and practical aspects of mechanical and software components.
  • Analysis of sorting statistics and their impact on speed and quality.
  • Consideration of cell preparation, viability, instrument setup, sort strategies, and biosafety.

Main Results:

  • High-speed sorting is enabled by advancements in high-speed electronics and data processing.
  • Optimal high-speed sorting requires precise implementation of established "slow-speed" sorting principles.
  • Multiple factors including cell preparation, instrument configuration, and sort strategy are critical.

Conclusions:

  • High-speed cell sorting is feasible with modern technology but demands meticulous attention to detail.
  • Understanding the interplay between hardware, software, and biological factors is key to successful high-speed sorting.
  • This work provides a framework for optimizing high-speed cell sorting protocols.