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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jun 17, 2026

Cost-Efficient Transcriptomic-Based Drug Screening
06:40

Cost-Efficient Transcriptomic-Based Drug Screening

Published on: February 23, 2024

RNA-Seq: a method for comprehensive transcriptome analysis.

Ugrappa Nagalakshmi1, Karl Waern, Michael Snyder

  • 1Molecular, Cellular, and Developmental Biology Department, Yale University, New Haven, Connecticut, USA.

Current Protocols in Molecular Biology
|January 14, 2010
PubMed
Summary
This summary is machine-generated.

RNA Sequencing (RNA-Seq) offers high-resolution transcriptome analysis by sequencing complementary DNAs (cDNAs). This method precisely quantifies transcripts and maps gene structures, surpassing older techniques.

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

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Last Updated: Jun 17, 2026

Cost-Efficient Transcriptomic-Based Drug Screening
06:40

Cost-Efficient Transcriptomic-Based Drug Screening

Published on: February 23, 2024

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Traditional sequencing methods like Sanger sequencing and microarrays have limitations in transcriptome analysis resolution.
  • RNA Sequencing (RNA-Seq) has emerged as a powerful technique for high-resolution genome-wide transcriptome analysis.

Purpose of the Study:

  • To describe protocols for performing RNA Sequencing (RNA-Seq).
  • To detail the application of RNA-Seq using the Illumina sequencing platform.

Main Methods:

  • RNA is converted to complementary DNAs (cDNAs).
  • cDNAs are sequenced using next-generation sequencing technologies.
  • Sequence reads are aligned to a reference genome to create a transcriptome map.

Main Results:

  • RNA-Seq enables precise quantification of transcript levels.
  • The technique accurately confirms or revises gene annotations, including 5' and 3' ends.
  • Exon/intron boundaries can be precisely mapped.

Conclusions:

  • RNA Sequencing (RNA-Seq) provides a high-resolution transcriptome analysis method.
  • Protocols for RNA-Seq on the Illumina platform are described.
  • This technique advances genomic and transcriptomic research capabilities.