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Updated: Jun 17, 2026

Generation of Recombinant Influenza Virus from Plasmid DNA
Published on: August 3, 2010
Human RNA polymerase I-driven reverse genetics for influenza a virus in canine cells
Pirada Suphaphiphat1, Bjoern Keiner, Heidi Trusheim
1Novartis Vaccines and Diagnostics, 45 Sidney Street, Cambridge, MA 02139, USA.
Abstract:
We have established a human RNA polymerase I (pol I)-driven influenza virus reverse genetics (RG) system in the Madin-Darby canine kidney 33016-PF cell line, which is approved for influenza vaccine manufacture. RNA pol I polymerases are generally active only in cells of species closely related to the species of origin of the polymerases. Nevertheless, we show that a nonendogenous RNA pol I promoter drives efficient rescue of influenza A viruses in a canine cell line. Application of this system allows efficient generation of virus strains and presents an alternative approach for influenza vaccine production.
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