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Related Concept Videos

Uncertainty in Measurement: Reading Instruments02:46

Uncertainty in Measurement: Reading Instruments

Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
Accuracy and Precision01:52

Accuracy and Precision

Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value.  Highly accurate measurements...
Uncertainty in Measurement: Accuracy and Precision03:37

Uncertainty in Measurement: Accuracy and Precision

Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value.
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Instrument Calibration01:12

Instrument Calibration

Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

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Updated: Jun 17, 2026

Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings
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Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings

Published on: February 26, 2016

Precision of Color Measurement with the GE Spectrophotometer. II: Photometric Accuracy.

E C Rhodes, F W Billmeyer

    Applied Optics
    |January 15, 2010
    PubMed
    Summary

    A calibrated phototube photometer achieved high absolute accuracy. This instrument verified the photometric accuracy of a General Electric recording spectrophotometer, confirming its reliable performance across its measurement range.

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    Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings
    08:33

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    Published on: February 26, 2016

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    Published on: May 12, 2012

    Area of Science:

    • Photometry
    • Spectrophotometry
    • Optical Measurement

    Background:

    • Accurate photometric measurements are crucial for scientific research and instrument calibration.
    • Existing calibration methods may have limitations in precision and scope.
    • The need for precise verification of spectrophotometer performance is essential.

    Purpose of the Study:

    • To calibrate a simple phototube photometer using Reule's supplementary light method.
    • To determine the absolute photometric accuracy of the calibrated photometer.
    • To assess the photometric accuracy of a General Electric recording spectrophotometer.

    Main Methods:

    • Application of Reule's supplementary light method for photometer calibration.
    • Utilizing a calibrated phototube photometer for photometric measurements.
    • Testing the photometric scale of a General Electric recording spectrophotometer.

    Main Results:

    • The phototube photometer was calibrated to an absolute accuracy of +/-0.006% (95% confidence limits).
    • The General Electric recording spectrophotometer demonstrated photometric accuracy within 0.1% over its entire range.
    • The calibrated photometer provided a reliable standard for spectrophotometer verification.

    Conclusions:

    • Reule's supplementary light method enables high-accuracy photometer calibration.
    • The calibrated photometer successfully validated the accuracy of the spectrophotometer.
    • Precise photometric calibration is achievable and vital for instrument validation.