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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
Mannosylated liposomes for targeted vaccines delivery.
Suresh Prasad Vyas1, Amit K Goyal, Kapil Khatri
1Department of Pharmaceutical Sciences, Drug Delivery Research Laboratory, Sagar, India.
Methods in Molecular Biology (Clifton, N.J.)
|January 15, 2010
Summary
Novel mannosylated liposomes enhance immune response by targeting dendritic cells and macrophages. O-palmitoyl mannan (OPM)-coated liposomes show superior uptake and cellular responses in vivo.
Area of Science:
- Nanotechnology
- Immunology
- Drug Delivery Systems
Background:
- Mannosylated liposomes are potential carriers for proteins, peptides, and nucleic acids.
- Targeting specific receptors on immune cells like dendritic cells and macrophages is crucial for effective antigen delivery.
Purpose of the Study:
- To describe novel mannosylated liposomes for enhanced intracellular targeting of immunogens.
- To evaluate the immune-stimulating activity and in vivo efficacy of these liposomal formulations.
Main Methods:
- Liposomes were prepared using a hand-shaken method.
- Characterization included size, shape, surface charge, encapsulation efficiency, and ligand binding.
- Immune response was assessed by measuring antigen-specific antibody titers and cellular responses in BALB/c mice.
Main Results:
- O-palmitoyl mannan (OPM)-coated liposomes demonstrated enhanced uptake efficiency.
- In vivo studies showed significantly higher serum antibody response with OPM-coated liposomes.
- Stronger TH1/TH2-based cellular responses were observed.
Conclusions:
- Novel mannosylated liposomes serve as effective nanosized carriers for antigen delivery.
- Superior surface characteristics facilitate interaction with antigen-presenting cells.
- These liposomes promote enhanced antigen processing and presentation, leading to a robust immune response.
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