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Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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Related Experiment Video

Updated: Jun 17, 2026

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
15:55

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Liposomal siRNA delivery.

Jeffrey Hughes1, Preeti Yadava, Ryan Mesaros

  • 1Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 15, 2010
PubMed
Summary
This summary is machine-generated.

Delivering small interfering RNA (siRNA) to the cell nucleus is crucial for gene silencing. Lipid/nucleic acid complexes offer a promising solution for effective delivery and protection.

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Related Experiment Videos

Last Updated: Jun 17, 2026

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy
15:55

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes
10:33

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches
08:26

Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

Published on: May 1, 2018

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Drug Delivery Systems

Background:

  • Small interfering RNA (siRNA) enables gene expression silencing.
  • Efficient delivery of siRNA to the cell nucleus is a significant challenge.
  • Lipid-based complexation is a key strategy for nucleic acid delivery.

Purpose of the Study:

  • To explore the formation and application of lipid/nucleic acid complexes for siRNA delivery.
  • To investigate methods for enhancing siRNA delivery efficiency and stability.
  • To evaluate the potential of lipid formulations in gene silencing applications.

Main Methods:

  • Utilizing electrostatic interactions between cationic lipids and anionic siRNA.
  • Engineering lipid vesicle size to influence tissue uptake.
  • Developing targeted lipoplexes for increased delivery efficiency.
  • Formulating lipid complexes to protect siRNA from immune system degradation.

Main Results:

  • Lipid/nucleic acid complex formation is a viable strategy for siRNA delivery.
  • Electrostatic interactions facilitate complex formation.
  • Liposome size and targeting moieties influence delivery and efficiency.
  • Formulation strategies can protect siRNA from degradation.

Conclusions:

  • Lipid/nucleic acid complexes represent a promising approach for intracellular siRNA delivery.
  • Optimization of lipid formulations and complex characteristics is key to successful gene silencing applications.
  • Further research into lipid-based delivery systems can advance RNA interference therapeutics.