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Updated: Jun 17, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Published on: February 7, 2019
The strength of transcription-factor binding modulates co-variation in transcriptional networks
Sergey V Nuzhdin1, Anna Rychkova, Matthew W Hahn
1University of Southern California, Program in Molecular and Computational Biology, Los Angeles, CA 90089, USA. snuzhdin@usc.edu <snuzhdin@usc.edu>
Abstract:
An appreciable fraction of the transcriptome differs in level of expression among individuals. Transcription factor (TF) expression and DNA binding causes cell-specific activation and repression of downstream targets, and TF expression levels vary across individuals. However, it is not clear how the strength of DNA binding for individual TFs translates into regulatory control, or whether a different set of binding motifs is used for strongly regulated modules. Here we integrate two publicly available data sets in Drosophila melanogaster, as well as conduct novel analyses, to address these questions.
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