Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 17, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Highly efficient gene transfer system using a laminin-DNA-apatite composite layer.

Ayako Oyane1, Hideo Tsurushima, Atsuo Ito

  • 1Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan. a-oyane@aist.go.jp

The Journal of Gene Medicine
|January 19, 2010
PubMed
Summary

Optimized laminin-DNA-apatite composite layers enhance gene transfer efficiency, particularly for nerve growth factor (NGF) delivery. This system successfully induced neuron-like differentiation in PC12 cells, showing promise for tissue engineering.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Basic Fibroblast Growth Factor-Releasing Bioabsorbable Polyglycolic Acid Dura Mater Enhances Neural Progenitor Cell Proliferation and Neuroprotection After Brain Injury.

Brain and behavior·2025
Same author

Polyethyleneimine-Assisted Fabrication of Poly(Lactic-Co-Glycolic Acid) Nanoparticles Loaded with Tamibarotene (Am80) for Meflin Expression Upregulation.

Journal of functional biomaterials·2025
Same author

Detection and Predictors of Arrhythmia in Patients with Chronic Noncardioembolic Ischemic Stroke on Wearable Electrocardiogram Device.

Asian journal of neurosurgery·2025
Same author

Integration of dual drugs into a collagen scaffold by a combination of apatite coating and impregnation with apatite particles for periodontal regeneration.

RSC advances·2025
Same author

One-Step Fabrication of Water-Dispersible Calcium Phosphate Nanoparticles with Immobilized Lactoferrin for Intraoral Disinfection.

International journal of molecular sciences·2025
Same author

Basic Fibroblast Growth Factor-releasing Polyglycolic Acid Duras Improve Neurological Function after Cerebral Infarction.

Current neurovascular research·2025

Area of Science:

  • Biomaterials Science
  • Molecular Biology
  • Cell Biology

Background:

  • A novel gene transfer system utilizing a laminin-DNA-apatite composite layer has been developed.
  • This system offers safety and efficiency for gene delivery applications.

Purpose of the Study:

  • To comprehensively characterize and optimize the laminin-DNA-apatite composite layer for enhanced gene transfer.
  • To evaluate the feasibility of this composite layer in inducing specific cell differentiation.

Main Methods:

  • Composite layers were prepared under diverse conditions and evaluated for gene transfer efficiency using luciferase and ss-galactosidase assays.
  • Optimized layers, incorporating nerve growth factor (NGF) cDNA, were applied to PC12 cells to assess neuron-like differentiation.

More Related Videos

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
07:14

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Published on: May 16, 2025

Related Experiment Videos

Last Updated: Jun 17, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids
07:14

Enhanced Gene Delivery and Expression using Intraosseous Injection of Chitosan Nanoparticles Encapsulated Adenine Base Editor Plasmids

Published on: May 16, 2025

Main Results:

  • Laminin content, not DNA content, was identified as the key factor enhancing gene transfer efficiency.
  • The cell adhesion properties of laminin were crucial for improved gene transfer, unlike albumin immobilization.
  • The optimized laminin-DNA-apatite composite layer successfully induced neuron-like differentiation in PC12 cells via NGF delivery.

Conclusions:

  • The developed gene transfer system demonstrates controllable efficiency and safety.
  • This system holds potential for applications in tissue engineering and the creation of transfection microarrays.
  • The ability to control cell differentiation makes this a valuable tool for advanced biological applications.