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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

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Related Experiment Video

Updated: Jun 17, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Alternative splicing: global insights.

Martina Hallegger1, Miriam Llorian, Christopher W J Smith

  • 1Department of Biochemistry, University of Cambridge, UK.

The FEBS Journal
|January 20, 2010
PubMed
Summary
This summary is machine-generated.

Alternative splicing allows genes to create multiple messenger RNA (mRNA) types, a process crucial for development and tissue regulation. New technologies are decoding this complex

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Related Experiment Videos

Last Updated: Jun 17, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

  • Molecular Biology
  • Genomics
  • Transcriptomics

Background:

  • Alternative splicing, the process of combining different splice sites, enables individual genes to produce multiple mRNA isoforms.
  • The prevalence of alternative splicing was underestimated until large-scale genome and transcriptome sequencing projects revealed it to be a widespread biological phenomenon.

Purpose of the Study:

  • To investigate the global scale and regulatory networks of alternative splicing.
  • To understand the 'RNA code' governing tissue- and developmentally regulated alternative splicing.
  • To explore how mutations can disrupt alternative splicing and lead to disease.

Main Methods:

  • Utilizing splice-sensitive microarray platforms for quantitative profiling of alternative splicing events.
  • Employing deep sequencing technologies for large-scale analysis of transcriptomes.
  • Conducting global analysis of RNA binding protein targets to map regulatory networks.
  • Applying sophisticated computational analysis to decipher splicing patterns.

Main Results:

  • Demonstrated that alternative splicing is a fundamental biological process, not an exception.
  • Enabled quantitative profiling of a vast number of alternative splicing events.
  • Revealed regulatory networks controlling post-transcriptional gene expression through RNA binding proteins.
  • Initiated the elucidation of the 'RNA code' for regulated splicing.

Conclusions:

  • Advanced technologies have revolutionized the study of alternative splicing, allowing for global-scale investigations.
  • Sophisticated computational and experimental approaches are key to understanding the 'RNA code'.
  • Disruptions in alternative splicing due to mutations are implicated in various diseases.