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Related Concept Videos

Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
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Related Experiment Video

Updated: Jun 16, 2026

Direct Reprogramming of Mouse Fibroblasts into Melanocytes
09:38

Direct Reprogramming of Mouse Fibroblasts into Melanocytes

Published on: August 27, 2021

Powering reprogramming with vitamin C.

Yan Shi1, Yang Zhao, Hongkui Deng

  • 1Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University, Shenzhen 518055, China.

Cell Stem Cell
|January 21, 2010
PubMed
Summary
This summary is machine-generated.

Vitamin C boosts the efficiency of reprogramming cells back into stem cells. This vitamin may help by reducing cell aging and working with epigenetic factors.

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Last Updated: Jun 16, 2026

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An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
11:53

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Published on: June 1, 2018

Area of Science:

  • Stem cell biology
  • Epigenetics
  • Cellular reprogramming

Background:

  • Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) is crucial for regenerative medicine.
  • Enhancing reprogramming efficiency remains a key challenge in the field.

Purpose of the Study:

  • To investigate the effect of vitamin C on the efficiency of cellular reprogramming.
  • To explore the mechanisms by which vitamin C might influence reprogramming.

Main Methods:

  • Fibroblasts from mice and humans were transduced with reprogramming factors (Oct4/Klf4/Sox2 and Oct4/Klf4/Sox2/cMyc).
  • Vitamin C was administered during the reprogramming process.
  • Cellular senescence and epigenetic modifications were analyzed.

Main Results:

  • Vitamin C significantly enhanced the reprogramming efficiency of both mouse and human fibroblasts.
  • Vitamin C demonstrated an ability to alleviate cell senescence, potentially through p53 repression.
  • The vitamin appeared to accelerate reprogramming, suggesting synergy with epigenetic regulators.

Conclusions:

  • Vitamin C is a potent enhancer of cellular reprogramming efficiency.
  • Vitamin C's beneficial effects may be linked to its anti-senescence properties and interaction with epigenetic mechanisms.
  • Further research into vitamin C's role could optimize iPSC generation for therapeutic applications.