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Updated: Jun 16, 2026

Evaluation of Caspase Activation to Assess Innate Immune Cell Death
Published on: January 20, 2023
Noxa is necessary for hydrogen peroxide-induced caspase-dependent cell death
Tomonori Aikawa1, Koei Shinzawa, Nobuyuki Tanaka
1Department of Medical Genetics, Osaka University Medical School, Osaka, Japan.
Abstract:
Oxidative stress induces apoptosis or necrosis of many cell types, which can cause tissue injury. Hydrogen peroxide (H(2)O(2)) induced apoptotic death of Jurkat cells. This effect was inhibited by overexpression of human Bcl-2, by silencing of cytochrome c, and by ablation of Bax/Bak, indicating that H(2)O(2)-induced apoptosis was mediated by the mitochondrial pathway in Jurkat cells. Treatment with H(2)O(2) caused an increase of Noxa protein, via activating transcription factor 4-dependent accumulation of Noxa mRNA and inhibition of Noxa protein degradation. H(2)O(2)-induced apoptosis was strongly suppressed by silencing of Noxa, indicating that Noxa plays a crucial role in this form of apoptosis.
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