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Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

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Updated: Jun 16, 2026

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
08:14

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Published on: October 28, 2014

Gene-delivery systems for iPS cell generation.

Lijian Shao1, Wen-Shu Wu

  • 1Maine Medical Center Research Institute, Maine Medical Center, COBRE in Stem Biology and Regenerative Medicine, 81 Research Drive, Scarborough, Maine 04074, USA.

Expert Opinion on Biological Therapy
|January 22, 2010
PubMed
Summary
This summary is machine-generated.

Induced pluripotent stem (iPS) cells hold promise for regenerative medicine. This review surveys gene-delivery systems for iPS cell generation, highlighting challenges in efficiency and genomic modification for clinical translation.

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Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
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Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

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Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
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Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

Related Experiment Videos

Last Updated: Jun 16, 2026

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
08:14

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

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Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

Published on: June 5, 2015

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Gene Therapy

Background:

  • Induced pluripotent stem (iPS) cells offer significant potential for regenerative medicine, disease modeling, and drug discovery.
  • Current iPS cell technology is still developing, with ongoing research focused on improving reprogramming efficiency and minimizing genomic alterations.
  • This review covers iPS cell reprogramming approaches and gene-delivery systems from 2006 to the present.

Purpose of the Study:

  • To provide a comprehensive survey of gene-delivery systems for generating iPS cells from somatic cells.
  • To categorize different gene-delivery vectors used in iPS cell reprogramming.
  • To discuss the advantages and limitations of these vectors for somatic cell reprogramming.

Main Methods:

  • Literature review of studies published between 2006 and the present.
  • Focus on gene-delivery systems and reprogramming approaches for iPS cell generation.
  • Categorization and analysis of gene-delivery vectors based on their characteristics.

Main Results:

  • Various gene-delivery vectors have improved iPS cell technology but still face challenges.
  • Current methods often suffer from low reprogramming efficiency or require multiple genomic modification steps.
  • Further research is needed to enhance existing vectors or develop novel ones for efficient human somatic cell reprogramming.

Conclusions:

  • A single, non-integrating reprogramming vector system is crucial for high efficiency.
  • Developing such a system is essential for generating clinically translatable human iPS cells.