Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Morphological and Elastic Transition of Polystyrene Adsorbed Layers on Silicon Oxide.

Journal of microscopy·2020

Same author

Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs.

Scientific reports·2018

Same author

Bio-functional surfaces for the immunocapture of AGO2-bound microRNAs.

Colloids and surfaces. B, Biointerfaces·2016

Same author

Adhesion mechanisms of the contact interface of TiO2 nanoparticles in films and aggregates.

Langmuir : the ACS journal of surfaces and colloids·2012

Same author

Predictors of clinical efficacy of 'Ablate and Pace' therapy in patients with permanent atrial fibrillation.

Heart (British Cardiac Society)·2011

Same author

Imaging with low-voltage scanning transmission electron microscopy: a quantitative analysis.

Ultramicroscopy·2011

Same journal

AFM-Modified Graphene Field-Effect Transistor for Sensitive Detection of Cardiac Troponin I.

Nanotechnology·2026

Same journal

Ultra-Sensitive UV Photodetectors Enabled by Built-in Electric Fields in Hierarchical NP-Type Porous Silicon.

Nanotechnology·2026

Same journal

Effect of sintering temperature on structural, microstructural and magnetic properties of La<sub>0.8</sub>Sr<sub>0.2</sub>MnO<sub>3</sub>: Evolution of faceting and terrace like morphology.

Nanotechnology·2026

Same journal

Engineered V2C MXene Anchored Cu Nanoparticles for Selective Nitrate/Nitrite Sensing and Magneto-Electrocatalytic Hydrogen Evolution Reaction.

Nanotechnology·2026

Same journal

Quantitative Mechanism Separation of Single-Event Transients in Nanosheet Transistors via TCAD Simulation.

Nanotechnology·2026

Same journal

Antibacterial, mechanical and curing properties of PMMA bone cement loaded with copper nanoparticles.

Nanotechnology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Published on: June 12, 2018

Scanning probe nanoimprint lithography.

F Dinelli¹, C Menozzi, P Baschieri

¹IPCF, Consiglio Nazionale delle Ricerche, CNR Campus, Via G. Moruzzi 1, Pisa PI 56100, Italy.

|January 22, 2010

Summary

This summary is machine-generated.

This study introduces a new nanoscale lithography method using scanning probe microscopy (SPM) and nanoimprint lithography (NIL). The technique enables real-time monitoring and control of the nanoimprint process for precise feature creation.

More Related Videos

Focused Ion Beam Lithography to Etch Nano-architectures into Microelectrodes

Focused Ion Beam Lithography to Etch Nano-architectures into Microelectrodes

Published on: January 19, 2020

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

Published on: January 23, 2013

Related Experiment Videos

Last Updated: Jun 16, 2026

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

Published on: June 12, 2018

Focused Ion Beam Lithography to Etch Nano-architectures into Microelectrodes

Focused Ion Beam Lithography to Etch Nano-architectures into Microelectrodes

Published on: January 19, 2020

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

Published on: January 23, 2013

Area of Science:

Materials Science
Nanotechnology
Surface Science

Background:

Conventional nanoimprint lithography (NIL) faces challenges in precise control and real-time monitoring.
Scanning probe microscopy (SPM) offers high-resolution imaging and manipulation capabilities at the nanoscale.

Purpose of the Study:

To develop a novel lithographic approach integrating SPM and NIL for enhanced nanoscale patterning.
To enable real-time monitoring and control of the nanoimprint process, including applied load and forces.
To demonstrate the capability of creating customized indenting tools for specific nanoscale feature fabrication.

Main Methods:

Utilized an atomic force microscope (AFM) integrated with custom software for precise load application and real-time monitoring.
Employed focused ion beam (FIB) sculpting to create customized AFM tips for nanoimprint lithography.
Applied anti-sticking layers to functionalize AFM tips for investigating indentation and de-molding effects.

Main Results:

Successfully imprinted features on a polystyrene NIL-patterned sample using controlled normal load and time intervals.
Demonstrated real-time monitoring of the imprint process on a thermoplastic polymer film.
Validated the effectiveness of customized AFM tips and anti-sticking layers in controlling nanoscale feature formation.

Conclusions:

The developed SPM-based NIL approach provides a novel and effective method for nanoscale lithography.
Real-time monitoring and controlled force application allow for precise fabrication of nanoscale features.
This technique offers a versatile platform for investigating and optimizing nanoimprint processes for various materials.