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Related Experiment Video

Updated: Jun 16, 2026

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

Published on: October 16, 2014

RNA interference in ageing research--a mini-review.

Nadège Minois¹, Peter Sykacek, Brian Godsey

¹Research Institute of Molecular Pathology/Institute of Molecular Biotechnology, Vienna, Austria.

|January 22, 2010

Summary

RNA interference (RNAi) is a powerful tool for studying gene function in ageing research, enabling the identification of new ageing-related genes and aiding in understanding age-related diseases. Advances in RNAi technology, including genome-wide screens, offer precise control for dynamic ageing process analysis.

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Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

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Published on: September 29, 2011

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Last Updated: Jun 16, 2026

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

Published on: October 16, 2014

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

Published on: September 29, 2011

Area of Science:

Genetics
Molecular Biology
Gerontology

Background:

Genetic mechanisms significantly influence lifespan and aging.
RNA interference (RNAi) is a key technique for gene silencing, crucial for identifying novel aging-related genes.
Genome-wide RNAi screens simplify the discovery of genes involved in aging.

Purpose of the Study:

To review RNAi applications in aging research, focusing on model organisms.
To highlight the impact of RNAi technology and recent developments in the field.
To discuss the potential of RNAi in identifying novel aging-related genes and understanding age-related diseases.

Main Methods:

RNA interference (RNAi) application to complement classic mutant studies.
Utilizing genome-wide RNAi screens in *Drosophila melanogaster* with a comprehensive transgenic RNAi library.
Employing flexible RNAi induction for studying dynamic processes and developing computational methods.

Main Results:

RNAi effectively complements traditional mutant studies in aging research.
Genome-wide RNAi screens in *D. melanogaster* offer novel opportunities for gene discovery.
Flexible RNAi induction aids in studying dynamic aging processes and gene interactions.

Conclusions:

RNAi is a powerful tool for characterizing gene roles in aging and identifying new implicated genes.
RNAi facilitates understanding age-related diseases by enabling manipulation of previously inaccessible genes.
Advanced RNAi techniques, including time-course experiments, allow high-resolution analysis of aging dynamics and gene interactions, paving the way for future research.