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Updated: Jun 16, 2026

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Optical path-length modulation for three-dimensional particle measurement in mirror-embedded microchannels.

Sungyoung Choi¹, Seung-Hoon Kim, Je-Kyun Park

¹Department of Bio and Brain Engineering, College of Life Science and Bioengineering, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea.

|January 22, 2010

Summary

Researchers developed a simple, low-cost method for 3D particle measurement in microfluidic devices using mirror-embedded channels and optical path-length modulation. This technique simplifies 3D imaging, overcoming limitations of conventional velocimetry methods.

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Area of Science:

Microfluidics
Optical Engineering
Particle Imaging

Background:

Conventional 3D particle velocimetry methods are complex, expensive, and require precise alignment.
Characterizing microfluidic devices with spatiotemporally varying 3D characteristics demands advanced measurement techniques.

Purpose of the Study:

To develop a simple, low-cost, and effective method for three-dimensional (3D) particle measurement in microfluidic channels.
To overcome the limitations of existing 3D particle imaging velocimetry and particle streak velocimetry.

Main Methods:

Implementation of mirror-embedded microchannels for reflecting side-view images.
Utilizing optical path-length (OPL) modulation with a high refractive index medium (cover glass) to offset optical path differences.

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Last Updated: Jun 16, 2026

A Protocol for Real-time 3D Single Particle Tracking

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Published on: January 3, 2018

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

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Acquiring two orthogonal-axis images (top and side views) for 3D positional information.

Main Results:

Successfully obtained 3D positional information of 6 µm-sized beads in microchannels.
Demonstrated simultaneous in-focus imaging of both side and top views.
Verified the 3D imaging principle in linear and grooved microchannel designs.

Conclusions:

The mirror-embedded microchannel scheme offers a readily fabricable and simple solution for 3D particle measurement.
This method significantly simplifies the implementation of 3D particle velocimetry in microfluidic research.
The technique is adaptable to existing microfluidic designs, promoting wider adoption.