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Microfluidic devices for cell based high throughput screening.

Sarvesh Upadhyaya1, P Ravi Selvaganapathy

  • 1Department of Mechanical Engineering, McMaster University, Hamilton, ON, Canada.

Lab on a Chip
|January 22, 2010
PubMed
Summary

Researchers developed a novel microfluidic device for precise, spatial dosing in cell-based assays. This new format overcomes miniaturization limits, enabling higher density and more efficient drug discovery screening.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Drug Discovery

Background:

  • Cell-based screening assays are vital in drug discovery, offering physiological relevance and high-content data.
  • Current microwell plate formats face limitations in miniaturization due to cell-wall interactions.
  • There is a need for advanced formats to enhance throughput and content in cell-based screening.

Purpose of the Study:

  • To introduce a novel microfluidic format for precise, spatially controlled dosing of continuous cell cultures.
  • To overcome the miniaturization limitations of current cell-based assay formats.
  • To enable higher density and more efficient high-throughput screening.

Main Methods:

  • A microfluidic device featuring a nanoporous membrane for spatially controlled chemical addressing.

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  • Application of external electric fields to precisely dose specific locations within a continuous gel layer.
  • Demonstration of dosing accuracy, spot size control, and inter-spot distances.

    • Main Results:

    • Achieved precise dosing with approximately 50 microgram accuracy.
    • Generated spot sizes ranging from 200 micrometers to 6 millimeters with inter-spot distances of 0.4-10 mm.
    • Obtained microarray spot densities of 156 spots/cm², five times higher than current assays.
    • Demonstrated successful handling of small molecules, proteins, and drugs.

    Conclusions:

    • The developed microfluidic spatial dosing format enables significant miniaturization of cell-based assays.
    • This technology facilitates high-throughput, high-content screening with enhanced precision and density.
    • The new format holds promise for advancing drug discovery and related biological research.