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Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
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B-cell-T-cell activation and interaction in common variable immunodeficiency.

Nima Rezaei1, James B Wing, Asghar Aghamohammadi

  • 1Department of Infection and Immunity, The University of Sheffield, Sheffield, UK. nima_rezaei@farabi.tums.ac.ir

Human Immunology
|January 26, 2010
PubMed
Summary
This summary is machine-generated.

Common variable immunodeficiency (CVID) impairs B-cell activation, leading to recurrent infections. This study found CVID patients have defective T-cell help for B cells or reduced B-cell responsiveness.

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Area of Science:

  • Immunology
  • Clinical Medicine
  • Cell Biology

Background:

  • Common variable immunodeficiency (CVID) is a primary immunodeficiency characterized by hypogammaglobulinemia.
  • Patients with CVID are prone to recurrent bacterial and viral infections due to impaired humoral immunity.
  • Understanding the cellular defects in CVID is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate B-cell and T-cell activation and interaction in patients with Common variable immunodeficiency (CVID).
  • To assess the role of T-cell help in B-cell activation in CVID patients.
  • To compare immune responses in CVID patients with age- and sex-matched healthy controls.

Main Methods:

  • Peripheral blood mononuclear cells (PBMCs) were isolated from 19 CVID patients and healthy controls.
  • In vitro assays were performed using polyclonal T-independent type 2 antigen mimic (anti-Ig-D-dextran) and polyclonal T-cell stimuli (anti-CD3/anti-CD28).
  • B-cell and T-cell activation markers, including CD86 expression, were analyzed.

Main Results:

  • T-cell activation and proliferation in response to anti-CD3/anti-CD28 stimulation were comparable between CVID patients and controls.
  • B-cell activation was significantly reduced in CVID patients when PBMCs were stimulated with polyclonal T-cell agonists alone.
  • Lower CD86 expression on B cells in CVID patients indicated impaired B-cell responses to T-cell help.

Conclusions:

  • The study suggests a defect in T-cell help provided to B cells, or a diminished B-cell response to T-cell help, in the studied CVID cohort.
  • These findings contribute to understanding the immunopathogenesis of Common variable immunodeficiency.
  • Further research is warranted to elucidate the specific mechanisms underlying this T-cell-B-cell interaction defect in CVID.