Related Experiment Video
Updated: Jun 16, 2026

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials
Published on: May 16, 2022
Effects and interactions of medium components on laccase from a marine-derived fungus using response surface
Donna D'Souza-Ticlo1, Sandeep Garg, Chandralata Raghukumar
1Marine Biotechnology Laboratory, National Institute of Oceanography, Council of Scientific & Industrial Research, Dona Paula, Goa, India.
Abstract:
The effects of various synthetic medium components and their interactions with each other ultimately impact laccase production in fungi. This was studied using a laccase-hyper-producing marine-derived basidiomycete, Cerrena unicolor MTCC 5159. Inducible laccases were produced in the idiophase only after addition of an inducer such as CuSO(4). Concentration of carbon and nitrogen acted antagonistically with respect to laccase production. A combination of low nitrogen and high carbon concentration favored both biomass and laccase production. The most favorable combination resulted in 917 U L(-1) of laccase. After sufficient growth had occurred, addition of a surfactant such as Tween 80 positively impacted biomass and increased the laccase activity to around 1,300 U L(-1). Increasing the surface to volume ratio of the culture vessel further increased its activity to almost 2,000 U L(-1).
Related Concept Videos
Methods of Medium Optimization
Response Surface Methodology
The process of RSM involves several key steps:
Factors Affecting Activity Coefficient
The activity coefficient value for an ion is close to one when the solution has almost zero ionic strength, i.e., when the solution shows close to ideal behavior. As the ionic strength of the solution increases from 0 to 0.1 mol/L, a decrease in the...
Production of Organic Acids
