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Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

A liquid chromatography to capillary array electrophoresis interface for two-dimensional separations.

Cameron D Skinner¹

¹Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke St West, Montreal, QC, Canada H4B 1R6. cskinner@alcor.concordia.ca

|January 26, 2010

Summary

This summary is machine-generated.

A novel liquid chromatography-capillary electrophoresis interface enables advanced multidimensional separations. This system utilizes multiple capillaries for enhanced sampling rates and diverse separation modes, improving analytical capabilities.

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

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Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Area of Science:

Analytical Chemistry
Separation Science
Biotechnology

Background:

Multidimensional separations are crucial for complex sample analysis.
Existing interfaces often limit sampling rates or separation versatility.
Need for improved interfaces in liquid chromatography-capillary electrophoresis (LC-CE).

Purpose of the Study:

To develop and demonstrate a new LC-CE interface for enhanced multidimensional separations.
To enable simultaneous or sequential multidimensional separations with different selectivities.
To facilitate sensitive detection of analytes in complex matrices.

Main Methods:

Development of a servo-controlled LC-CE interface with multiple second-dimension capillaries.
Utilizing gel filtration for the first dimension and sub-micellar capillary array electrophoresis for the second dimension.
Employing a polydimethylsiloxane (PDMS) multichannel sheath-flow cuvette for laser-induced fluorescence detection.

Main Results:

Successful implementation of a multidimensional separation of fluorescently labeled serum.
Demonstration of simultaneous collection of two different multidimensional separations by varying background electrolytes.
Achieved sensitive detection of labeled serum components using the developed interface and detection system.

Conclusions:

The new LC-CE interface significantly advances multidimensional separation capabilities.
The system offers flexibility for higher sampling rates and diverse separation strategies.
The developed interface and detection method are suitable for sensitive analysis of complex biological samples.