A threshold LC-MS/MS method for 92 analytes in oral fluid collected with the Quantisal® device.
Brigitte Desharnais1, Marie-Jo Lajoie2, Julie Laquerre2
1Laboratoire de sciences judiciaires et de médecine légale, Department of Toxicology, 1701 Parthenais Street, Montréal, Québec, H2K 3S7, Canada; Concordia University, Department of Chemistry & Biochemistry, 7141 Sherbrooke Street West, Montréal, Québec, H4B 1R6, Canada.
Forensic Science International
|October 9, 2020
Summary
A new qualitative method was developed to detect 97 drugs and metabolites in oral fluid (OF) following cannabis legalization. This validated method successfully screens 92 analytes, aiding impaired driving research.
Area of Science:
- Forensic Toxicology
- Analytical Chemistry
Background:
- Cannabis legalization in Canada necessitates robust methods for impaired driving detection.
- Oral fluid (OF) analysis is a viable alternative to blood testing for drug monitoring.
Purpose of the Study:
- To develop and validate a qualitative decision point method for detecting 97 drugs and metabolites in OF.
- To prepare for a study on impaired driving rates in Québec post-cannabis legalization.
Main Methods:
- A high-throughput qualitative method using Quantisal® devices for OF collection.
- Incubation with a precipitation solvent (acetone:acetonitrile 30:70 v:v) to enhance drug recovery and stability.
- Validation included testing for interferences, carryover, stability, recovery, reproducibility, robustness, and accuracy.
Main Results:
- The validated method covers 92 analytes, with 5 additional analytes screened.
- Five analytes (olanzapine, oxazepam, 7-aminoclonazepam, flunitrazepam, nitrazepam) were monitored but did not meet all validation criteria.
- Successful blind internal proficiency testing demonstrated the method's reliability.
Conclusions:
- A validated qualitative OF method is established for monitoring numerous drugs and metabolites.
- This method supports research into impaired driving following recreational cannabis legalization.
- The method demonstrates high throughput and reliability for forensic toxicology applications.
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