Related Experiment Video
Updated: Jun 16, 2026
08:59
Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Chitosanase displayed on liposome can increase its activity and stability
Kien Xuan Ngo1, Hiroshi Umakoshi, Toshinori Shimanouchi
1Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
Journal of Biotechnology
|January 27, 2010
Summary
Researchers developed a novel biocatalyst by immobilizing chitosanase onto liposomes (ICL). This immobilized enzyme exhibits enhanced activity and stability compared to conventional chitosanase, offering a promising new method for enzyme immobilization.
Area of Science:
- Biocatalysis and Enzyme Engineering
- Biotechnology and Nanomaterials
Background:
- Enzyme immobilization is crucial for enhancing biocatalyst stability and reusability.
- Liposomes offer a versatile platform for enzyme encapsulation and delivery.
- Chitosanase plays a key role in chitin degradation, with applications in various industries.
Purpose of the Study:
- To develop a novel biocatalyst by immobilizing chitosanase onto liposomes (ICL).
- To characterize the immobilized chitosanase (ICL) in terms of protein characteristics, activity, and stability.
- To investigate the influence of liposome surface properties on the immobilized enzyme's performance.
Main Methods:
- Immobilization of chitosanase onto liposomes via direct interaction with Streptomyces griseus cell membrane.
- Characterization using SDS-PAGE to determine protein molecular weight.
- Assay of chitosanase activity and evaluation of stability under various temperatures and pH conditions.
Main Results:
- SDS-PAGE confirmed the presence of a ~39 kDa protein in ICL, corresponding to mature chitosanase and its signal peptide.
- Immobilized chitosanase (ICL) demonstrated significantly higher chitosanase activity compared to the conventional enzyme.
- ICL exhibited enhanced stability under various temperatures and pH conditions, highlighting the importance of liposome surface hydration.
Conclusions:
- The direct immobilization of chitosanase onto liposomes creates a highly active and stable biocatalyst (ICL).
- Liposome surface properties critically influence the activity of the immobilized chitosanase.
- This liposome-based immobilization strategy provides a robust method for preparing advanced biocatalysts.
Related Concept Videos
01:05Bioavailability Enhancement: Drug Stability Enhancement and GI Retention
Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
01:16Bioavailability Enhancement: Drug Solubility Enhancement
Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
01:27Bioavailability Enhancement: Drug Permeability Enhancement
After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt secretion,...
01:06Lipid Digestion
Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
01:20Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry
Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...