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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

High-resolution light microscopy using luminescent nanoparticles.

Tymish Y Ohulchanskyy1, Indrajit Roy, Ken-Tye Yong

  • 1Institute for Lasers, Photonics and Biophotonics, SUNY at Buffalo, Buffalo, NY, USA. tyo2@buffalo.edu

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
|January 27, 2010
PubMed
Summary
This summary is machine-generated.

This review highlights advancements in luminescent nanoparticles for advanced microscopy. It covers silica, gold, quantum dots, and nanophosphors, detailing their pros, cons, and applications in bioimaging.

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Area of Science:

  • Nanotechnology
  • Biomedical Imaging
  • Materials Science

Background:

  • Confocal and multiphoton microscopy require advanced imaging probes.
  • Luminescent nanoparticles offer unique optical properties for enhanced imaging.
  • Developing novel nanoprobes is crucial for high-resolution biological studies.

Purpose of the Study:

  • To review recent developments in luminescent nanoparticles for microscopy.
  • To discuss various classes of nanomaterials used as imaging nanoprobes.
  • To evaluate the advantages, disadvantages, and applications of these nanoparticles.

Main Methods:

  • Review of recent scientific literature on luminescent nanoparticles.
  • Categorization of nanomaterials into four main classes: silica-based, gold, semiconductor nanocrystals (quantum dots/rods), and nanophosphors.
  • Discussion of specific advanced nanoprobes, including ORMOSIL nanoparticles and lanthanoid-doped nanophosphors.

Main Results:

  • Silica-based nanoparticles offer tunable fluorescence.
  • Gold nanoparticles provide unique scattering and plasmonic properties.
  • Semiconductor nanocrystals (quantum dots) exhibit bright, photostable emission.
  • Nanophosphors demonstrate long-lasting luminescence and potential for multiplexing.
  • Organically modified silica (ORMOSIL) nanoparticles show aggregation-enhanced fluorescence (AEF).

Conclusions:

  • Luminescent nanoparticles are versatile tools for advanced microscopy.
  • Each class of nanomaterial offers distinct advantages for specific bioimaging applications.
  • Emerging nanoprobes like ORMOSIL and nanophosphors show significant promise for future research.