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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
Published on: January 7, 2017
A carbon nanotubes based ATP apta-sensing platform and its application in cellular assay
Libing Zhang1, Hui Wei, Jing Li
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Biosensors & Bioelectronics
|January 29, 2010
Summary
A new fluorescent aptasensor detects adenosine triphosphate (ATP) with high sensitivity. This method utilizes dye-labeled aptamers and carbon nanotubes for sensitive ATP detection in biological samples.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Nanotechnology
Background:
- Adenosine triphosphate (ATP) is a crucial molecule in cellular energy transfer and signaling.
- Accurate detection of ATP is vital for understanding various biological processes and diseases.
- Existing ATP detection methods often lack sensitivity, selectivity, or require complex procedures.
Purpose of the Study:
- To develop a highly sensitive and selective fluorescent aptasensor for adenosine triphosphate (ATP) detection.
- To demonstrate the utility of the aptasensor for detecting ATP in complex biological samples.
Main Methods:
- Construction of a fluorescent aptasensor based on the noncovalent assembly of dye-labeled ATP aptamer and single-walled carbon nanotubes (SWNTs).
- Utilized the fluorescence quenching effect of SWNTs on the dye in the absence of ATP.
- Observed fluorescence enhancement upon ATP binding to the aptamer, displacing SWNTs.
Main Results:
- The developed aptasensor achieved a sensitive detection limit for ATP down to 4.5 nM.
- Demonstrated high selectivity for ATP detection.
- Obtained prominent fluorescence signals in cellular ATP assays, indicating applicability in real samples.
Conclusions:
- The novel fluorescent aptasensor represents the most sensitive fluorescent ATP aptasensor reported to date.
- The aptasensor is capable of detecting ATP in biological samples, offering a promising tool for biomedical research.
- This method provides a sensitive, selective, and potentially cost-effective approach for ATP quantification.
