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Accuracy and precision of HDL cholesterol measurements using an office chemistry analyzer
Background:
A physician can obtain a patient's complete lipoprotein profile at the time of the office visit including assays of the total serum cholesterol, high-density lipoprotein cholesterol (HDL-C), and fasting triglyceride concentrations, and then calculate the low-density lipoprotein cholesterol (LDL-C). Until recently, this was not possible. Instruments are currently available that provide reliable rapid total serum cholesterol and fasting triglyceride measurements.
Methods:
This study evaluated the accuracy and precision of a recently developed analytical method for the rapid measurement of HDL-C (Seralyzer Cholesterol System) as compared with a reference clinical laboratory method (Kodak Ektachem 700 XR). Blood specimens were taken from 90 participants and were analyzed in duplicate for HDL-C concentrations and total cholesterol using the Seralyzer and a standard Ektachem 700 XR.
Results:
Nearly all (98.9%) of the initial Seralyzer HDL-C measures were within +/- 0.08 mmol/L (+/- 3 mg/dL) of the duplicate Seralyzer values. Most (98.3%) of the Seralyzer HDL-C results were within +/- 0.16 mmol/L (+/- 6 mg/dL) of the Kodak HDL-C values.
Conclusions:
The Seralyzer HDL-C test provides a reliable and accurate measure of the HDL-C concentration.