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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jun 16, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Splicing factor and exon profiling across human tissues.

Pierre de la Grange1, Lise Gratadou, Marc Delord

  • 1GenoSplice technology, Centre Hayem, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75010, Paris, France. didier.auboeuf@inserm.fr

Nucleic Acids Research
|January 30, 2010
PubMed
Summary
This summary is machine-generated.

Alternative splicing is more common in certain human tissues like the cerebellum, testis, and spleen. This prevalence is linked to higher expression of genes, including splicing factors, in these specific tissues.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Last Updated: Jun 16, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Published on: June 24, 2021

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Area of Science:

  • Molecular Biology
  • Genomics
  • Transcriptomics

Background:

  • Alternative splicing is a key mechanism for generating protein diversity.
  • Previous studies indicated higher prevalence of alternative splicing in brain and testis.
  • The underlying reasons for tissue-specific differences in alternative splicing remain incompletely understood.

Purpose of the Study:

  • To investigate the propensity of specific human tissues to generate splicing variants.
  • To identify the relationship between splicing factor expression and alternative splicing prevalence across tissues.
  • To provide a comprehensive resource on expression profiling of alternative splicing and splicing factors.

Main Methods:

  • Utilized high-density microarray data for expression profiling.
  • Analyzed splicing factor and exon expression across 11 normal human tissues.
  • Performed exon-based statistical group analysis and extensive RT-PCR validation.

Main Results:

  • Cerebellum, testis, and spleen showed the highest proportion of differentially expressed alternative exons.
  • Higher alternative exon variation correlated with increased expression of splicing factors in these tissues.
  • The observed splicing factor expression pattern mirrored global gene expression patterns in these tissues.

Conclusions:

  • The higher prevalence of alternative splicing in cerebellum, testis, and spleen is attributed to a greater number of expressed genes, including splicing factors.
  • This study offers a valuable dataset for understanding tissue-specific alternative splicing.
  • Gene expression levels significantly influence the extent of alternative splicing in human tissues.