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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: Jun 16, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Target-enrichment strategies for next-generation sequencing.

Lira Mamanova1, Alison J Coffey, Carol E Scott

  • 1The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.

Nature Methods
|January 30, 2010
PubMed
Summary

Target enrichment is crucial for sequencing specific DNA regions when whole genome sequencing isn't feasible. This study details leading technologies, optimizations, and protocols to guide researchers in selecting the best approach for their projects.

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Last Updated: Jun 16, 2026

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Whole genome sequencing of eukaryotes is not yet routine.
  • Target enrichment is necessary to select and amplify specific genomic regions before sequencing.
  • Various target enrichment technologies exist, each with distinct pros and cons.

Purpose of the Study:

  • To describe experiences with leading target enrichment technologies.
  • To detail optimizations performed on these technologies.
  • To provide typical results and detailed protocols for end users.

Main Methods:

  • Evaluation of leading target enrichment technologies.
  • Optimization of protocols for selected technologies.
  • Analysis of obtained sequencing results.

Main Results:

  • Comparative analysis of different target enrichment methods.
  • Demonstration of optimized protocols yielding specific results.
  • Presentation of typical data quality metrics (sensitivity, specificity, uniformity).

Conclusions:

  • Target enrichment is a vital technique in genomics.
  • Understanding technology-specific performance is key for project success.
  • Provided protocols enable users to balance sensitivity, specificity, and uniformity for their needs.