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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jun 16, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

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Published on: January 3, 2016

Angle scintillations in the laser return from a retroreflector.

J P Hansen, S Madhu

    Applied Optics
    |January 30, 2010
    PubMed
    Summary

    Laser return angle scintillations were observed to be eight times greater than expected due to atmospheric turbulence. This study presents experimental findings and theoretical analysis of this laser beam scintillation phenomenon.

    Area of Science:

    • Optics
    • Atmospheric Physics
    • Laser Technology

    Background:

    • Atmospheric turbulence significantly affects laser beam propagation.
    • Retroreflector systems are sensitive to environmental conditions impacting laser return signals.

    Purpose of the Study:

    • To investigate and quantify angle scintillations in laser return signals exceeding expected atmospheric turbulence effects.
    • To develop a theoretical model explaining the observed enhanced scintillation.

    Main Methods:

    • Experimental setup using a pulsed 1.06-micrometer laser and a corner cube retroreflector.
    • Measurement of angular scintillations of the laser return.
    • Comparison with a reference beacon to quantify scintillation magnitude.

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    Published on: June 6, 2017

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    Last Updated: Jun 16, 2026

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
    15:06

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    Published on: January 3, 2016

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    10:42

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    Published on: March 22, 2019

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    06:55

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    Published on: June 6, 2017

    Main Results:

    • Observed angular scintillations eight times greater than a reference beacon.
    • Experimental data indicates scintillation exceeding predictions based solely on atmospheric turbulence.
    • A theoretical analysis was performed to understand the underlying mechanism.

    Conclusions:

    • Atmospheric turbulence alone does not fully explain the observed laser return angle scintillations.
    • Phase fluctuations in the illuminating laser beam are proposed as a significant contributing factor.
    • A derived expression relates scintillation angle to phase fluctuation parameters.