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Updated: Jun 16, 2026

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
Published on: May 28, 2021
Cryogenic confocal microscopy with rotation in a magnetic field
T Kehoe1, M Ediger, R T Phillips
1Cavendish Laboratory, University of Cambridge, J. J. Thomson Avenue, Cambridge CB3 0HE, United Kingdom.
Abstract:
Two approaches to conducting magneto-optical confocal spectroscopy are described, in each of which the confocal head is rotatable with respect to the magnetic field. A coudé arrangement has been shown to give adequate performance for scanned imaging, but lower optical throughput than a system based on single-mode optical fiber transport. The design criteria for a cryogenic fiber-coupling objective are described, and the tolerances demanded in lens alignment are shown to be relatively benign, allowing manufacture without special techniques. The practical use of the rotating confocal system with commercial stick-slip positioners has been shown to be rigid enough, and asymmetric weight distribution and diamagnetic forces are small enough to permit single quantum emitters to be studied over a range of angles and field strengths.
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