Related Experiment Video
Updated: Jun 16, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Gibberellic acid and cGMP-dependent transcriptional regulation in Arabidopsis thaliana
René Bastian1, Adam Dawe, Stuart Meier
1Department of Biotechnology, University of the Western Cape, Bellville, South Africa.
Abstract:
An ever increasing amount of transcriptomic data and analysis tools provide novel insight into complex responses of biological systems. Given these resources we have undertaken to review aspects of transcriptional regulation in response to the plant hormone gibberellic acid (GA) and its second messenger guanosine 3',5'-cyclic monophosphate (cGMP) in Arabidopsis thaliana, both wild type and selected mutants. Evidence suggests enrichment of GA-responsive (GARE) elements in promoters of genes that are transcriptionally upregulated in response to cGMP but downregulated in a GA insensitive mutant (ga1-3). In contrast, in the genes upregulated in the mutant, no enrichment in the GARE is observed suggesting that GARE motifs are diagnostic for GA-induced and cGMP-dependent transcriptional upregulation. Further, we review how expression studies of GA-dependent transcription factors and transcriptional networks based on common promoter signatures derived from ab initio analyses can contribute to our understanding of plant responses at the systems level.
Related Concept Videos
Activation and Inactivation of G Proteins
Cell Signaling in Plants
Cell Specific Gene Expression
Global Regulatory Systems
Gene Regulation During Sporulation
GPCRs Regulate Adenylyl Cylase Activity
Two...

