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Related Concept Videos

Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jun 16, 2026

Whole-cell Patch-clamp Recordings in Brain Slices

Whole-cell Patch-clamp Recordings in Brain Slices

Published on: June 15, 2016

In-cell recordings by extracellular microelectrodes.

Aviad Hai¹, Joseph Shappir, Micha E Spira

¹Department of Neurobiology, Life Science Institute, the Hebrew University of Jerusalem, Jerusalem, Israel.

|February 2, 2010

Summary

This summary is machine-generated.

Researchers developed a novel

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Related Experiment Videos

Last Updated: Jun 16, 2026

Whole-cell Patch-clamp Recordings in Brain Slices

Whole-cell Patch-clamp Recordings in Brain Slices

Published on: June 15, 2016

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Published on: September 5, 2015

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

Published on: September 10, 2013

Area of Science:

Neuroscience
Biomedical Engineering
Cellular Biology

Background:

Extracellular recordings in neuroscience often have low signal-to-noise ratios.
This limitation restricts monitoring to action potentials and prevents subthreshold synaptic potential detection.

Purpose of the Study:

To develop a new method for high-fidelity neuronal recordings.
To overcome the limitations of current extracellular recording techniques.

Main Methods:

Inducing Aplysia californica neurons to actively engulf protruding microelectrodes.
Implementing 'in-cell recordings' for neuronal activity monitoring.

Main Results:

Achieved signal-to-noise ratios comparable to conventional intracellular recordings.
Enabled detection of both subthreshold synaptic and action potentials.

Conclusions:

The 'in-cell recording' technique offers a significant advancement in neuronal signal acquisition.
This method has the potential to enhance neuroscience research and biomedical applications.