Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanistic insights into lenacapavir-induced off-pathway HIV-1 capsid assembly.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Assessing the validity of leucine zipper constructs predicted by AlphaFold.

Protein science : a publication of the Protein Society·2025
Same author

IHMValidation: Assessment of Integrative Structure Models Deposited to the Protein Data Bank.

Journal of molecular biology·2025
Same author

The intersection of viral mimicry and nuclear entry.

Trends in biochemical sciences·2025
Same author

Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data.

IUCrJ·2024
Same author

IHMCIF: An Extension of the PDBx/mmCIF Data Standard for Integrative Structure Determination Methods.

Journal of molecular biology·2024

Related Experiment Video

Updated: Jun 16, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Small-angle scattering for structural biology--expanding the frontier while avoiding the pitfalls.

David A Jacques1, Jill Trewhella

  • 1School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, New South Wales 2006, Australia.

Protein Science : a Publication of the Protein Society
|February 2, 2010
PubMed
Summary
This summary is machine-generated.

Small-angle scattering (SAS) is increasingly used for studying biological macromolecules. This review provides guidelines for evaluating SAS data quality and modeling to ensure reliable protein structure and function insights.

More Related Videos

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution
12:53

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Published on: January 8, 2013

Related Experiment Videos

Last Updated: Jun 16, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution
12:53

Small and Wide Angle X-Ray Scattering Studies of Biological Macromolecules in Solution

Published on: January 8, 2013

Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Small-angle scattering (SAS) is a powerful technique for analyzing biological macromolecules in solution.
  • Its application has expanded beyond biophysics to protein science due to improved instrumentation and software.

Purpose of the Study:

  • To provide a guide for small-angle scattering experiments.
  • To establish guidelines for critical evaluation of scattering data and modeling assumptions.
  • To highlight best practices for advancing protein structure and function studies.

Main Methods:

  • Review of small-angle scattering principles and applications.
  • Guidelines for assessing sample and data quality.
  • Recommendations for 3D model calculations and interpretation.

Main Results:

  • Increased utility of SAS for protein structure and interaction studies.
  • Identification of challenges related to publication standards and data interpretation.
  • Demonstration of SAS power in advancing understanding of protein structure and function.

Conclusions:

  • Understanding sample/data quality and modeling is crucial for reliable SAS results.
  • Standardized evaluation of SAS data is needed.
  • SAS is a valuable technique for protein scientists to study structure and function.