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Updated: Jun 16, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
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Multiple serial picture presentation with millisecond resolution using a three-way LC-shutter-tachistoscope.

Florian Ph S Fischmeister1, Ulrich Leodolter, Christian Windischberger

  • 1MR Centre of Excellence, Medical University of Vienna, Lazarettgasse 14, A-1090 Vienna, Austria. florian.fischmeister@meduniwien.ac.at

Journal of Neuroscience Methods
|February 4, 2010
PubMed
Summary

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Researchers developed a new tachistoscope for precise control of visual stimulus presentation. This device enhances reliability in studying unconscious visual processing and general vision research.

Area of Science:

  • Cognitive Neuroscience
  • Visual Perception
  • Experimental Psychology

Background:

  • Growing interest in unconscious visual processing.
  • Concerns regarding the reliability of existing stimulus presentation devices.
  • Variability in display parameters affects visual stimulus processing.

Purpose of the Study:

  • To present a custom-built, reliable tachistoscope for precise stimulus delivery.
  • To enable millisecond resolution in experimental setups.
  • To overcome limitations of current visual presentation devices.

Main Methods:

  • Construction of a three-way LC-shutter-tachistoscope using LCD projectors and liquid crystal shutters.
  • Stimulus presentation via a mirror system onto a back projection screen.

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  • Empirical verification using photoreceiver measurements of optical power.

    • Main Results:

    • Demonstrated marked reduction in interfering variabilities of stimulus parameters.
    • Confirmed precise and reliable stimulus delivery with millisecond resolution.
    • Highlighted the device's flexibility and ease of setup.

    Conclusions:

    • The custom-built tachistoscope offers a highly flexible and reliable tool for vision research.
    • It is particularly suitable for studying unconscious visual processing.
    • Improves experimental control over stimulus presentation parameters.