Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flame Photometry: Overview01:02

Flame Photometry: Overview

Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
Flame Photometry: Lab01:16

Flame Photometry: Lab

In a flame photometer, when a solution like potassium chloride is aspirated into the flame, the solvent evaporates, leaving behind dehydrated salt. This salt dissociates into free gaseous atoms in their ground state. Some of these atoms absorb energy from the flame, leading to their excitation. The excited atoms return to the ground state, emitting photons at characteristic wavelengths. Because only electronic transitions are involved, the resulting emission lines are very narrow. The intensity...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Radiation standards.

Applied optics·2010
Same author

Reflectance measurements on cavity radiators.

Applied optics·2010
Same author

Mercury versus disposable thermometers.

Professional nurse (London, England)·1997
Same author

You are going SI, nurse! 10. Can you metricate?

Nursing mirror and midwives journal·1975
Same author

You are going SI, nurse! 9. Electricity and SI.

Nursing mirror and midwives journal·1975
Same author

[THE OCCLUSIVE ARTERIAL SYNDROME OF THE LOWER EXTREMITY AND TREATMENT BY LUMBAR SYMPATHECTOMY. REVIEW OF 315 CASES].

Angiologia·1965
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

New sector-disk method for comparing light intensities.

C Forno, E J Giliham

    Applied Optics
    |February 4, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel method using dual rotating sector disks to precisely reduce light intensity. This technique accurately measures light intensity ratios and filter transmissions, overcoming detector nonlinearity issues for reliable optical measurements.

    More Related Videos

    Method for Recording Broadband High Resolution Emission Spectra of Laboratory Lightning Arcs
    07:51

    Method for Recording Broadband High Resolution Emission Spectra of Laboratory Lightning Arcs

    Published on: August 27, 2019

    Surface Mapping of Earth-like Exoplanets using Single Point Light Curves
    06:48

    Surface Mapping of Earth-like Exoplanets using Single Point Light Curves

    Published on: May 10, 2020

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    Method for Recording Broadband High Resolution Emission Spectra of Laboratory Lightning Arcs
    07:51

    Method for Recording Broadband High Resolution Emission Spectra of Laboratory Lightning Arcs

    Published on: August 27, 2019

    Surface Mapping of Earth-like Exoplanets using Single Point Light Curves
    06:48

    Surface Mapping of Earth-like Exoplanets using Single Point Light Curves

    Published on: May 10, 2020

    Area of Science:

    • Optics and Photonics
    • Photometry and Radiometry

    Background:

    • Accurate measurement of light intensity is crucial in various scientific and industrial applications.
    • Traditional methods using sector disks can be limited by detector nonlinearity.
    • Talbot's law is a principle governing the behavior of light under certain conditions.

    Purpose of the Study:

    • To describe a new method for precise light attenuation using two rotating sector disks.
    • To enable accurate measurement of intensity ratios and filter transmissions.
    • To address and overcome errors caused by detector nonlinearity.

    Main Methods:

    • Utilizing two precisely rotating sector disks to attenuate a light beam by an exact integral factor.
    • Developing an apparatus to test if a photodetector adheres to Talbot's law.
    • Comparing the novel method with the ordinary sector-disk method.

    Main Results:

    • The described method allows for accurate intensity ratio and filter transmission measurements.
    • The method effectively eliminates errors arising from detector nonlinearity, provided Talbot's law is obeyed.
    • The new technique offers advantages over conventional sector-disk methods.

    Conclusions:

    • The dual rotating sector disk method provides a robust solution for accurate optical measurements.
    • This method is particularly valuable in applications requiring high precision and where detector linearity is a concern.
    • The developed apparatus aids in validating detector performance for this technique.