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Related Experiment Video

Updated: Jun 16, 2026

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

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Published on: May 3, 2013

Microscope with enhanced depth of field and 3-d capability.

J S Courtney-Pratt, R L Gregory

|February 4, 2010

Summary

Researchers developed a novel microscope objective that enhances depth of field by focusing spectral colors at different distances. This innovation enables sharp 3-D image displays with adjustable depth magnification.

Area of Science:

Microscopy
Optical Engineering
3-D Imaging

Background:

Traditional microscope objectives aim for achromatism, focusing all spectral colors at a single point.
Increasing the depth of field in microscopy is crucial for detailed 3-D visualization.

Purpose of the Study:

To explore a novel approach for increasing the effective depth of field in microscopy.
To enable 3-D image viewing by utilizing differential spectral color focusing.

Main Methods:

Designed a microscope objective that intentionally deviates from achromatism, causing spectral colors to focus at different distances.
Modified a binocular eyepiece to adjust image convergence based on spectral color.
Developed a 16-mm NA 0.4 objective achieving 1-micrometer lateral resolution and 100-micrometer depth of field.

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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

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Main Results:

Demonstrated a microscope objective with an extended depth of field.
Achieved a lateral resolution of 1 micrometer and a depth of field of 100 micrometers.
Developed 3-D displays capable of presenting sharp images with variable depth magnification up to +/- 40,000x.

Conclusions:

The proposed method effectively increases the depth of field in microscopy.
This technique allows for high-resolution 3-D imaging with adjustable depth magnification.
The developed objective and display system offer significant advancements in microscopic visualization.