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Updated: Jun 16, 2026

Isolation and Characterization of Microvesicles from Peripheral Blood
06:03

Isolation and Characterization of Microvesicles from Peripheral Blood

Published on: January 6, 2017

Microfluidic isolation and transcriptome analysis of serum microvesicles.

Chihchen Chen1, Johan Skog, Chia-Hsien Hsu

  • 1BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Lab on a Chip
|February 4, 2010
PubMed
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Researchers developed a rapid microfluidic method to isolate microvesicles (exosomes) from blood. This technique simplifies biomarker discovery for cancer diagnosis and prognosis, offering clinical potential.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Microvesicles (exosomes) are crucial for intercellular communication, carrying biomarkers from host cells.
  • Analysis of microvesicles in blood shows potential for cancer biomarker discovery.
  • Current isolation methods (centrifugation, filtration) are time-consuming and complex.

Purpose of the Study:

  • To develop a faster and simpler method for isolating microvesicles from biological samples.
  • To enable efficient extraction of high-quality RNA from isolated microvesicles for genetic analysis.
  • To facilitate the use of microvesicles as clinical biomarkers for cancer diagnosis and prognosis.

Main Methods:

  • A microfluidic immunoaffinity technique was developed for microvesicle isolation.

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Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

Related Experiment Videos

Last Updated: Jun 16, 2026

Isolation and Characterization of Microvesicles from Peripheral Blood
06:03

Isolation and Characterization of Microvesicles from Peripheral Blood

Published on: January 6, 2017

Isolation of Small Noncoding RNAs from Human Serum
06:44

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

  • The method was tested using small volumes of blood serum and cell culture medium.
  • High-quality RNA was successfully extracted from the isolated microvesicles.

    • Main Results:

    • The microfluidic method provides an easy and rapid isolation of microvesicles.
    • It allows for the extraction of RNA suitable for genetic analysis.
    • This facilitates the potential use of microvesicles as cancer biomarkers.

    Conclusions:

    • The developed microfluidic immunoaffinity method offers a significant improvement over existing techniques.
    • This advancement supports the clinical utility of microvesicles as biomarkers for cancer.
    • It paves the way for improved cancer diagnosis and prognosis through genetic profiling.