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Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
Alfredo Gragnani1, Marina Bertelli Rossi, Janne Cely Medeiros Albuquerque
1Plastic Surgery Division, UNIFESP, Sao Paulo, Brazil. gragnani.dcir@epm.br
Purpose:
To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes.
Methods:
Oxidative stress was produced through addition of hydrogen peroxide (H(2)O(2)) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H(2)O(2) (G H(2)O(2)), Group H(2)O(2) and KGF (G H(2)O(2)-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay.
Results:
The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001).
Conclusion:
KGF determined protection to the primary human keratinocytes exposed to oxidative stress.
