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Updated: Jun 16, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Semi-selective broth improves screening for methicillin-resistant Staphylococcus aureus.

S Böcher¹, B Middendorf, H Westh

¹National Center for Antimicrobials and Infection Control, Statens Serum Institut, Copenhagen, Denmark.

The Journal of Antimicrobial Chemotherapy

|February 5, 2010

Summary

A new semi-selective enrichment broth (TSB-SSI) significantly improved methicillin-resistant Staphylococcus aureus (MRSA) detection compared to direct plating and non-selective broth. This advancement enhances routine diagnostic screening for MRSA.

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Last Updated: Jun 16, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Area of Science:

Clinical Microbiology
Infectious Diseases
Diagnostic Laboratory Science

Background:

Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant public health threat, necessitating accurate and efficient diagnostic methods.
Current diagnostic approaches for MRSA, including direct plating and non-selective enrichment broths, may have limitations in sensitivity and specificity.
Optimizing enrichment broths can enhance the detection rates of MRSA in clinical samples.

Purpose of the Study:

To evaluate the performance of two enrichment broths for the detection of MRSA.
To compare the efficacy of these broths against direct plating methods.
To assess the potential for improving routine MRSA diagnostic screening.

Main Methods:

Analysis of 1224 patient swabs using six different methods in a central laboratory.
Inoculation onto ChromID MRSA agar and Columbia sheep blood agar (BA) after incubation in non-selective broth (NB) or a semi-selective broth (TSB-SSI) containing cefoxitin and aztreonam.
Comparison of results from enrichment broths with direct plating and initial peripheral laboratory findings.

Main Results:

The semi-selective broth (TSB-SSI) detected the highest number of MRSA isolates (n=223), outperforming non-selective broth (n=205) and direct plating (n=203).
TSB-SSI significantly reduced the growth of methicillin-susceptible Staphylococcus aureus (MSSA), improving the clarity of results.
While overall sensitivity was 85%, the study indicated improved MRSA detection with TSB-SSI compared to other methods, even with pre-used swabs.

Conclusions:

The semi-selective enrichment broth (TSB-SSI) demonstrated superior performance for MRSA detection compared to non-selective broth and direct plating.
Routine diagnostic screening for MRSA can be significantly enhanced by employing TSB-SSI.
The study highlights the value of optimized enrichment broths in improving clinical microbiology diagnostics.