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OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
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Engineering an artificial zymogen by alternate frame protein folding.

Diana M Mitrea1, Lee S Parsons, Stewart N Loh

  • 1Department of Biochemistry & Molecular Biology, State University of New York Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA.

Proceedings of the National Academy of Sciences of the United States of America
|February 6, 2010
PubMed
Summary

Alternate frame folding (AFF) introduces allostery into proteins. This study engineered barnase into a zymogen activated by HIV-1 protease, demonstrating AFF

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