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Updated: Jun 16, 2026

Detection of Functional Matrix Metalloproteinases by Zymography
Published on: November 8, 2010
Assay of matrix metalloproteinases against matrix substrates
Tim E Cawston1, Rachel L Lakey, Andrew D Rowan
1Musculoskeletal Research Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
Abstract:
The assays described allow the activity of members of the matrix metalloproteinase (MMP) family that degrade collagen, gelatin and casein substrates to be measured. The protocols described include the preparation of radiolabeled substrates, methods for the separation of degraded product from undegraded substrate, and methods for the activation of MMPs. The advantages and disadvantages of these methods are discussed in relation to immunoassays that measure the amount of individual MMPs.
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