Recommendations for fluorescence instrument qualification: the new ASTM Standard Guide
Paul C DeRose1, Ute Resch-Genger
1National Institute of Standards and Technology, 100 Bureau Dr., Gaithersburg, Maryland 20899-8312, USA. paul.derose@nist.gov
Analytical Chemistry
|February 9, 2010
Summary
ASTM International is releasing a Standard Guide for Fluorescence to enhance quality assurance in fluorometry. This guide provides procedures for instrument characterization and performance verification, aiding fluorescence measurement standardization.
Area of Science:
- Analytical Chemistry
- Spectroscopy
Background:
- Modern fluorescence techniques require robust quality assurance and accurate quantitation.
- Standardization is crucial for reliable and reproducible fluorescence measurements across different laboratories and instruments.
Purpose of the Study:
- To review the upcoming ASTM International Standard Guide for Fluorescence.
- To highlight the guide's focus on steady-state fluorometry and instrument characterization.
- To emphasize the guide's role in improving fluorescence measurement standardization.
Main Methods:
- Discussion of available standards and instrument characterization procedures for steady-state fluorometry.
- Identification of key instrument properties requiring qualification (e.g., linearity, spectral responsivity, wavelength accuracy).
- Brief introduction to procedures for determining fluorescence quantum yields and lifetimes.
Main Results:
- The guide details essential instrument properties for qualification and performance verification.
- Procedures are adaptable to various fluorescence techniques beyond steady-state fluorometry.
- The guide offers instructions for use, suitability, and purpose of discussed standards.
Conclusions:
- The ASTM Standard Guide for Fluorescence aims to improve quality assurance and quantitation in fluorescence techniques.
- It serves as a reference for users of fluorescence instrumentation, promoting standardization.
- The guide addresses critical aspects of instrument performance and measurement reliability.
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