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Updated: Jun 16, 2026
An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
Published on: November 3, 2010
Errors and reproducibility of DNA array-based detection of allelic variants in ADME genes: PHARMAchip
Elisabet Cuyàs1, Estibaliz Olano-Martín, Olha Khymenets
1Human Pharmacology & Clinical Neurosciences Research Group of the Neuropsychopharmacology Program, Institut Municipal d'Investigació Mèdica (IMIM-Hospital del Mar), Parc de Recerca Biomèdica de Barcelona, c/ Dr. Aiguader 88/08003 Barcelona, Spain. rtorre@imim.es
Aims:
Differences in adverse drug reactions can be explained by genetic variations, especially if they determine the expression of certain protein effectors and/or drug-metabolizing enzymes. Over the last decade, several tests screening for the most frequent polymorphisms in drug-metabolizing enzymes have been marketed for research and diagnostic purposes. The aim of this study was to assess the suitability of PHARMAchip for the genotyping of polymorphisms of genes associated with drug metabolism and response as an alternative to Jurilab Ltd's DrugMEt Test.
Materials & Methods:
In this observational study, performed using 100 previously genotyped DNA samples, we report on common genes included in the two different tests examined: the former DrugMEt test and the recently introduced PHARMAchip test.
Results & Conclusion:
Although these tests are based on different methodological approaches, we have found a high concordance of results between both methods. Some of the discrepancies between tests were related to allelic variants not monitored in a particular microarray and the quality of the genomic DNA used.
