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Cloning of the gene encoding an endo-acting pectate lyase from Streptomyces thermocarboxydus
Akio Tonouchi1, Yuichi Hara, Ryousuke Umehara
1Faculty of Agriculture and Life Science, Hirosaki University, Aomori, Japan. symbio@cc.hirosaki-u.ac.jp
Bioscience, Biotechnology, and Biochemistry
|February 9, 2010
Abstract:
An endo-acting family 3 pectate lyase (PL I) gene from Streptomyces thermocarboxydus was cloned and expressed in Escherichia coli. The deduced PL I sequence had highest similarity to a putative pectate lyase of S. coelicolor. Recombinant PL I had significantly lower specific activity, probably a result of incorrect folding, as indicated by circular dichroism spectra analysis.
