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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

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Related Experiment Video

Updated: Jun 16, 2026

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

A nonviral minicircle vector for deriving human iPS cells.

Fangjun Jia1, Kitchener D Wilson, Ning Sun

  • 1Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA.

Nature Methods
|February 9, 2010
PubMed
Summary
This summary is machine-generated.

Researchers generated transgene-free induced pluripotent stem cells (iPSCs) using minicircle DNA. This nonviral method avoids insertional mutagenesis risks associated with viral vectors for iPSC generation.

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Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
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Related Experiment Videos

Last Updated: Jun 16, 2026

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Published on: January 1, 2017

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
12:03

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Published on: October 31, 2012

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
08:14

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Published on: October 28, 2014

Area of Science:

  • Stem Cell Biology
  • Genetic Engineering
  • Molecular Biology

Background:

  • Viral transduction for induced pluripotent stem cells (iPSCs) carries insertional mutagenesis risks.
  • Nonviral methods are increasingly preferred for safer iPSC generation.

Purpose of the Study:

  • To evaluate minicircle DNA as a nonviral vector for generating transgene-free iPSCs.
  • To demonstrate the efficacy of a single minicircle vector for iPSC production.

Main Methods:

  • Utilized minicircle DNA, a bacterial DNA-free vector with high cellular expression.
  • Applied a single minicircle vector for reprogramming adult human adipose stem cells.

Main Results:

  • Successfully generated transgene-free induced pluripotent stem cells (iPSCs).
  • Demonstrated the effectiveness of minicircle DNA in generating iPSCs from adipose stem cells.

Conclusions:

  • Minicircle DNA is a viable and safe nonviral alternative for generating transgene-free iPSCs.
  • This method mitigates risks associated with traditional viral transduction techniques.