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Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Published on: January 1, 2017
A nonviral minicircle vector for deriving human iPS cells
Fangjun Jia1, Kitchener D Wilson, Ning Sun
1Departments of Medicine and Radiology, Stanford University School of Medicine, Stanford, California, USA.
None:
Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem cells (iPSCs). We report the use of 'minicircle' DNA, a vector type that is free of bacterial DNA and capable of high expression in cells, for this purpose. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells.
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