Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advances in Biomaterial-Based Cardiac Organoids Engineering for Modeling Development and Congenital Heart Defects.

ACS applied bio materials·2026

Same author

Author Correction: Plasma membrane curvature regulates the formation of contacts with the endoplasmic reticulum.

Nature cell biology·2026

Same author

Cardiac-Derived ECM Microspheres for Enhanced hiPSC-CMs Maturation.

Advanced functional materials·2026

Same author

<i>OrgaCast</i>: A Trustworthy Spatiotemporal Diffusion Model for Fluorescence Organoid Forecasting.

Proceedings of the ... AAAI Conference on Artificial Intelligence. AAAI Conference on Artificial Intelligence·2026

Same author

A Deep Learning-Based Scoring Framework for Large-Scale Multi-Donor Cardiotoxicity Screening.

bioRxiv : the preprint server for biology·2026

Same author

A Generative AI Framework to Predict Cardiomyocyte Contraction Function from Single Static Images.

bioRxiv : the preprint server for biology·2026

Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026

Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026

Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026

Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026

Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026

Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Dec 23, 2025

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Published on: April 3, 2020

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils.

Soah Lee¹, Huaxiao Yang¹, Caressa Chen¹

¹Stanford Cardiovascular Institute, Stanford University School of Medicine; Department of Medicine, Division of Cardiovascular Medicine, Stanford University; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University.

Journal of Visualized Experiments : Jove

|April 21, 2020

Summary

This summary is machine-generated.

A new lithography-free method uses stencils for single-cell patterning, enabling precise control over cell shape and size. This accessible technique facilitates cell fate studies for researchers with limited bioengineering backgrounds.

More Related Videos

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

Related Experiment Videos

Last Updated: Dec 23, 2025

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Published on: April 3, 2020

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

Area of Science:

Cell Biology
Biotechnology
Biomaterials Science

Background:

Micropatterning is crucial for studying cell fate determination at the single-cell level.
Existing methods like soft lithography require specialized engineering skills and facilities.

Purpose of the Study:

To introduce a simple, accessible, lithography-free method for single-cell patterning.
To overcome the limitations of current micropatterning techniques.

Main Methods:

Developed a stencil-based single-cell patterning technique.
Detailed procedures for stencil design, hydrogel fabrication, protein incorporation, and cell culture.
Demonstrated patterning of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).

Main Results:

Successfully patterned up to 2,000 cells with defined shapes.
Achieved distinct cell shapes, from squares to adult cardiomyocyte-like rectangles.
Showcased the ability to control cell morphology for hiPSC-CMs.

Conclusions:

The stencil-based method is robust, convenient, inexpensive, and accessible.
This technique provides a valuable tool for cell biology research, particularly for those with limited bioengineering expertise.
Facilitates precise control over cell shape and size for single-cell studies.