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Related Experiment Video

Updated: Jun 16, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Single molecule sensitive multivalent polyethylene glycol probes for RNA imaging.

Aaron W Lifland¹, Chiara Zurla, Philip J Santangelo

¹Georgia Institute of Technology and Emory University, Atlanta, Georgia.

Bioconjugate Chemistry

|February 10, 2010

Summary

We developed a sensitive RNA imaging probe for live cells. This probe accurately visualizes human respiratory syncytial virus (hRSV) RNA using novel delivery methods with rapid binding kinetics.

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Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

Published on: June 21, 2019

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Last Updated: Jun 16, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method

Published on: June 21, 2019

Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

Imaging RNA in live cells is challenging due to probe delivery and sensitivity limitations.
Probes require precise cellular compartment delivery and rapid, sensitive binding to detect low RNA levels.

Purpose of the Study:

To characterize a novel, single-molecule sensitive, multivalent RNA imaging probe.
To evaluate the probe's efficacy in live cells for imaging human respiratory syncytial virus (hRSV) genomic RNA.

Main Methods:

Utilized an eight-armed poly(ethylene glycol) core for probe construction.
Delivered the probe into live cells via reversible membrane permeabilization and TAT-peptide mediated transduction.
Assessed binding kinetics and visualized subcellular RNA distributions and RNA-protein colocalization.

Main Results:

The probe achieved single-molecule sensitivity for RNA imaging.
Accurate imaging of hRSV genomic RNA was demonstrated in live cells.
Both delivery methods bypassed the endosomal pathway and showed binding kinetics under 10 minutes.
Subcellular RNA localization and RNA-protein colocalization were successfully visualized.

Conclusions:

The developed multivalent RNA probe enables sensitive, accurate live-cell imaging of viral RNA.
Novel delivery methods offer efficient intracellular access and rapid probe-target interaction.
This technology advances the study of viral RNA dynamics and interactions within host cells.