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Updated: Jun 16, 2026
Grossing of Non-neoplastic Globes, Including Fetal Eyes
Published on: May 30, 2025
Harvesting fresh tumor tissue from enucleated eyes: the 2008 Jack S. Guyton lecture
Jerry A Shields1, Carol L Shields, Sara E Lally
1Ocular Oncology Service, Wills Eye Institute, 840 Walnut St, Ste 1440, Philadelphia, PA 19107, USA. jerry.shields@shieldsoncology.com
Abstract:
There is an increasing need to obtain fresh tumor tissue and normal tissue for genetic studies and research following enucleation of an eye for uveal melanoma, retinoblastoma, and other lesions. A concern about doing this is that the globe will often collapse and lead to difficulties with gross and histopathologic interpretation. The technique reported herein is based on the creation of an opening in the sclera using an 8-mm corneal trephine followed by removal of tumor tissue. This method provides adequate tissue, preserves the spherical configuration of the eye, and allows for excellent histopathologic sections.
Insights
Obtaining fresh ocular tissues for research after eye removal can be challenging. A novel technique using an 8-mm corneal trephine preserves globe structure, yielding adequate tissue for analysis.
Area of Science:
- Ophthalmology
- Oncology
- Surgical Pathology
Background:
- Increasing demand for fresh ocular tumor and normal tissue for genetic research.
- Globe collapse post-enucleation complicates gross and histopathologic interpretation.
Purpose of the Study:
- To present a novel technique for obtaining fresh ocular tissue after enucleation.
- To address challenges in tissue preservation and interpretation.
Main Methods:
- Enucleation of the eye for lesions like uveal melanoma and retinoblastoma.
- Creation of a scleral opening using an 8-mm corneal trephine.
- Removal of tumor tissue through the trephine opening.
Main Results:
- Preservation of the eye's spherical configuration.
- Adequate fresh tissue yield for genetic studies and research.
- Facilitation of excellent histopathologic sections.
Conclusions:
- The described trephine technique effectively overcomes globe collapse.
- This method ensures high-quality tissue for ocular oncology research.
- Improved histopathologic interpretation is achieved with preserved globe integrity.
