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Published on: December 16, 2021
MicroRNA155 is induced in activated CD4(+) T cells of TNBS-induced colitis in mice
Da-Fan Chen1, Bang-Dong Gong, Qing Xie
1Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin Second Road, Shanghai 200025, China.
Aim:
To investigate the expression of microRNA155 (miRNA155) in trinitrobenzene sulphonic acid (TNBS)-induced colitis and the relationship between miRNA155 and tumor necrosis factor (TNF) expressions.
Methods:
In TNBS colitis mice, miRNA155 and TNF mRNA expressions were measured in colons and CD4(+) T cells of draining lymph nodes (LNs). CD4(+) T cells were cultured in vitro with or without anti-CD3/CD28 antibody, and the expressions of miRNA155 and TNF mRNA in cells and TNF concentration in culture media were examined.
Results:
miRNA155 and TNF mRNA expressions in colons and in cells of LNs were significantly increased in TNBS colitis compared with controls. In TNBS colitis, miRNA155 and TNF mRNA expressions in CD4(+) T cells of LNs and TNF concentration in CD4(+) T cells culture media increased compared with controls. When cultured with anti-CD3/CD28 antibody, miRNA155 and TNF mRNA expressions in CD4(+) T cells and TNF concentration in the CD4(+) T cells culture media were significantly higher than those cultured without anti-CD3/CD28 antibody. Following analysis using the Pearson's correlation coefficient, miRNA155 expression had a significant positive correlation with either TNF mRNA expression in CD4(+) T cells (r = 0.860, P < 0.05) or TNF concentration in CD4(+) T cells culture media (r = 0.892, P < 0.05).
Conclusion:
miRNA155 is induced in colons and activated CD4(+) T cells in TNBS colitis, and the levels of miRNA155 and TNF expressions have a significant positive correlation.
Insights
MicroRNA155 (miRNA155) and tumor necrosis factor (TNF) expressions are elevated in trinitrobenzene sulphonic acid (TNBS)-induced colitis. Increased miRNA155 correlates positively with TNF levels in activated CD4(+) T cells.
Area of Science:
- Immunology
- Molecular Biology
- Gastroenterology
Background:
- Inflammatory bowel diseases (IBD) involve complex immune dysregulation.
- MicroRNAs (miRNAs) play critical roles in regulating gene expression during inflammation.
- Tumor necrosis factor (TNF) is a key pro-inflammatory cytokine implicated in IBD pathogenesis.
Purpose of the Study:
- To investigate microRNA155 (miRNA155) expression in trinitrobenzene sulphonic acid (TNBS)-induced colitis.
- To determine the relationship between miRNA155 and tumor necrosis factor (TNF) expression in this colitis model.
Main Methods:
- TNBS-induced colitis model in mice.
- Quantification of miRNA155 and TNF mRNA in colon tissues and CD4(+) T cells.
- In vitro culture of CD4(+) T cells with or without anti-CD3/CD28 stimulation.
- Measurement of miRNA155, TNF mRNA, and TNF protein levels in cultured cells and media.
Main Results:
- Significantly increased miRNA155 and TNF mRNA expression in colons and lymph node CD4(+) T cells of TNBS-treated mice compared to controls.
- Elevated miRNA155 and TNF mRNA in CD4(+) T cells, and increased TNF concentration in culture media, observed in TNBS colitis.
- Upregulation of miRNA155 and TNF in CD4(+) T cells upon in vitro activation.
- Significant positive correlation (r = 0.860 to 0.892, P < 0.05) between miRNA155 expression and TNF levels.
Conclusions:
- miRNA155 is induced in the colon and activated CD4(+) T cells during TNBS-induced colitis.
- A strong positive correlation exists between miRNA155 and TNF expression levels in this inflammatory model.
- miRNA155 may play a role in regulating TNF-mediated inflammation in colitis.
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